Journal: Vaccines

Article Title: Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals

doi: 10.3390/vaccines10020332

Figure Lengend Snippet: Humoral and cellular immune responses in SARS-CoV-2-recovered individuals. ( a ) SARS-CoV-2 S1 IgG ELISA ratio; ( b ) pVNT 50 titer; ( c ) IFN-γ ELISpot counts from visit one to visit four for unvaccinated and vaccinated individuals. The arrow indicates the vaccination time in the single-dose-vaccinated group. ( d ) SARS-CoV-2 S1 IgG ELISA ratio; ( e ) pVNT 50 titer; ( f ) IFN-γ ELISpot counts from visit one to visit four in unvaccinated individuals from the longitudinal study. ( g ) SARS-CoV-2 S1 IgG ELISA ratio; ( h ) pVNT 50 titer; ( i ) IFN-γ ELISpot counts from visit one to visit four in single-dose-vaccinated individuals from the longitudinal study. The blue dashed line indicates the cutoff (S1-IgG = 0.8 for negative and 1.1 for positive, pVNT 50 = 10). The significance of the differences between the unvaccinated and single-dose-vaccinated groups was determined using two-way ANOVA by Holm–Šídák’s multiple comparisons test. ns, p > 0.05, **** p < 0.0001.

Article Snippet: The inhibition rate (%) of samples was calculated as follows: 1 − mean RLU of the sample - mean RLU of the negative control mean RLU of the positive control - mean RLU of the negative control × 100 Then, 50% of the SARS-CoV-2 pseudovirus-neutralizing antibody titer (pVNT 50 ) was determined by non-linear regression using GraphPad Prism 9.3.0 (GraphPad Software, Inc., San Diego, CA, USA). pVNT 50 below the detection limit was recorded as 10 and was considered the cutoff titer.

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot

Journal: Vaccines

Article Title: Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals

doi: 10.3390/vaccines10020332

Figure Lengend Snippet: Humoral and cellular immune responses in SARS-CoV-2-recovered individuals vaccinated with a single- or double-dose COVID-19 vaccine. ( a ) SARS-CoV-2 S1 IgG ELISA ratio; ( b ) pVNT 50 titer; ( c ) IFN-γ ELISpot counts. The blue dashed line indicates the cutoff (S1-IgG = 0.8 for negative and 1.1 for positive, pVNT 50 = 10). p values were determined using the Mann–Whitney test. ns, p > 0.05, * p < 0.05.

Article Snippet: The inhibition rate (%) of samples was calculated as follows: 1 − mean RLU of the sample - mean RLU of the negative control mean RLU of the positive control - mean RLU of the negative control × 100 Then, 50% of the SARS-CoV-2 pseudovirus-neutralizing antibody titer (pVNT 50 ) was determined by non-linear regression using GraphPad Prism 9.3.0 (GraphPad Software, Inc., San Diego, CA, USA). pVNT 50 below the detection limit was recorded as 10 and was considered the cutoff titer.

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot, MANN-WHITNEY

Journal: Vaccines

Article Title: Humoral and Cellular Responses to COVID-19 Vaccines in SARS-CoV-2 Infection-Naïve and -Recovered Korean Individuals

doi: 10.3390/vaccines10020332

Figure Lengend Snippet: Pre- and post-vaccination humoral and cellular immunities of SARS-CoV-2-naïve participants. ( a ) SARS-CoV-2 S1 IgG ELISA; ( b ) pVNT 50 titer; ( c ) IFN-γ ELISpot count. Wilcoxon matched-pairs signed-rank test was used to compare differences between each pre- and post-vaccination. The blue dashed line indicates the cutoff (S1-IgG = 0.8 for negative and 1.1 for positive, pVNT 50 = 10). Kruskal–Wallis test by Dunn’s multiple comparisons test was used to compare differences between the vaccine groups. ns, p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: The inhibition rate (%) of samples was calculated as follows: 1 − mean RLU of the sample - mean RLU of the negative control mean RLU of the positive control - mean RLU of the negative control × 100 Then, 50% of the SARS-CoV-2 pseudovirus-neutralizing antibody titer (pVNT 50 ) was determined by non-linear regression using GraphPad Prism 9.3.0 (GraphPad Software, Inc., San Diego, CA, USA). pVNT 50 below the detection limit was recorded as 10 and was considered the cutoff titer.

Techniques: Enzyme-linked Immunosorbent Assay, Enzyme-linked Immunospot